Cutting-Edge Flow Cytometry and Mass Spectrometry
Our flow cytometry core offers internal and external users a wide array of cytometers for accurate and reliable cell analysis, including fully sterile cell sorting for therapeutic applications. Users can obtain high-parametric data with our suite of full spectrum analyzers, cell sorters, and CyTOF Helios mass cytometer. Our experienced and knowledgeable staff offers in-depth, hands-on training and consultation to all core users.
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Schedule With Us
To schedule services with Flow Cytometry, or to get more information about how we can work with you, contact our labs:
Phone (Thomas Building): 206.667.6195
Phone (Steam Plant Building): 206.667.1221
We have a broad range of advanced cell analyzers from BD Biosciences. All our cell analyzers can acquire data from tubes and multi-well plates, with the option of high-throughput analysis.
Our team can provide you with consultation, training, assay development or assisted sessions.
The flow cytometry core has expanded our full spectrum analyzers and cell sorters with the industry's most advanced systems. We have cytometers from BD Biosciences, Cytek, and Sony capable of supporting high-parameter panels with more than 50 fluorescent markers. Including our new BD FACSDiscover S8 with CellView technology. Our team can provide you with consultation, training, assay development or assisted sessions.
We offer advanced cell sorters from BD Biosciences, Sony Biotechnology and Miltenyi Biotec that allow for live-cell sorting of a variety of cell types.
Cell sorting is considered a laboratory procedure hazard due to its potential for aerosol production. All sorters are housed in Class IIA biosafety cabinets to reduce the risk of exposure. However, pathogens designated as Risk Group 2 (RG2) require approval from the Institutional Biosafety Committee and will be approved on a case-by-case basis. Any sample exposed to agents higher than RG2 may not be handled in this facility unless fixed by methods verified as effective against the infectious agent.
The Sony ID 7000F is a high-parameter spectral cell analyzer with seven lasers and 186 detectors, making it one of the most advanced cytometers in the world. By collecting the entire spectrum of light from 360 nm to 920 nm, this cutting-edge cytometer allows for accurate detection of dim and rare cell populations with high sensitivity.
The BD FACSymphony A5 is an innovative and customized cell analyzer with five lasers allowing up to 28 colors simultaneously. This cytometer enables users to analyze complex fluorochrome panels with high accuracy. Two of our instruments, Symphony 3 and Symphony 5, can also be configured for spectral analysis (SE), which allows for more flexibility in your panel design.
Download more information on our Symphony 1 (Thomas Building)
Download more information on our Symphony 2 (Thomas Building)
Download more information on our Symphony 3 (Steam Plant Building)
Download more information on our Symphony 4 (Steam Plant Building)
Download more information on our Symphony 5 (Thomas Building)
The BD LSRFortessa X-50 is an innovative and customized cell analyzer with five lasers allowing up to 28 colors simultaneously. This specialized cytometer also can be configured for spectral analysis, which allows for more flexibility in your panel design.
The BD FACSCelesta is a custom cell analyzer with four lasers allowing up to 16 colors simultaneously. This powerful, compact instrument allows for a large panel while maintaining ease of use.
The BD FACSymphony A3 is a five laser cytometer allowing users to run up to 26 colors.
The Cytek Aurora is a full spectrum high parameter cell analyzer equipped with 5 lasers and 64 detectors. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless of assay complexity.
The BD FACSDiscoverTM S8 with CellViewTM image technology is the first spectral flow cytometer sorter with sort-capable image analysis, expanding the power of cell analysis and sorting to new dimensions by combining spectral flow cytometry with real-time spatial and morphological insights—empowering scientists to address previously impossible-to-answer questions. Please contact our team to learn more about how to design panels to best utilize the full instrument capabilities.
The BD FACSymphony S6 is an innovative and customized cell sorter with five lasers allowing up to 28 colors simultaneously. This cell sorter allows for six-way sorting and into variously sized tubes and multi-well plates. This device has spectral capabilities, please contact our team for more information.
The Sony MA900 is a cell sorter is an innovative and intuitive cell sorter with four lasers allowing up to 12 colors simultaneously. This cell sorter is notable for its ease of use even for less experienced users.
The Miltenyi Biotec MACSQuant Tyto is a cell sorter with three lasers and 10 detectors allowing for 8 color detection. This unique instrument allows users to quickly and gently sort cells in a fully sterile environment.
The Fluidigm Helios CyTOF mass spectrometry system enables analysis of more than 40 cell surface and intracellular markers without the spectral overlap seen in conventional flow cytometers. Learn more by downloading the user guide below.
Data Analysis
The Flow Cytometry core does not provide licenses for data analysis software packages, such as FlowJo. For Fred Hutch employees, FlowJo and Cytek SpectroFlo is available on the shared computer workstation at both the Steam Plant and Thomas flow cytometry core locations. Licenses for FlowJo can be obtained through Scientific Computing for use on personal computers.